Key Takeaways
- •Peptide arrays allow researchers to screen hundreds to thousands of peptides simultaneously on a single membrane.
- •For most screening applications, the standard array format uses small spots with a diameter between 1 and 2 mm.
- •High-density arrays are ideal when you need to test a large library of peptide sequences in a single experiment.
Peptide arrays allow researchers to screen hundreds to thousands of peptides simultaneously on a single membrane. The format of the array depends directly on the purpose of the screening. Understanding the available spot sizes, peptide counts, and membrane dimensions helps you design an experiment that fits your research goals.
Standard Array Format: Small Spots for High Density
For most screening applications, the standard array format uses small spots with a diameter between 1 and 2 mm. This compact spot size enables the synthesis of up to 1,400 peptides per membrane. The maximum membrane size for this format is about 8 cm x 13 cm.
High-density arrays are ideal when you need to test a large library of peptide sequences in a single experiment. The small spot size conserves membrane space while still providing reliable signal detection in binding or activity assays.
Large Spot Format for Solution Assays
When peptides are intended for solution-phase assays, a different array format is used. In this case, the spots are synthesized with a larger diameter of 5 to 7 mm. Because each spot occupies more area, the membrane can accommodate approximately 110 peptides per membrane.
Large-spot arrays are better suited for assays where the peptide must be cleaved from the membrane and used in solution, or when higher peptide yield per spot is required for downstream applications such as ELISA or functional screening.
Maximum Array Size and Customization
Regardless of the format chosen, the maximum array size is approximately 8 cm x 13 cm. This dimension applies to both small-spot and large-spot configurations. Researchers can customize the array layout to match their specific screening needs, including the arrangement of control peptides and replicates.
Custom peptide arrays are synthesized on cellulose membranes using SPOT synthesis technology. This method allows for rapid, parallel synthesis of many peptides directly on the support without the need for individual resin handling.
How to Request a Quote for a Peptide Array
To obtain a custom quote for a peptide array, you will need to provide details about your project. The quote request form typically requires information such as the number of peptides, desired spot size, membrane dimensions, and any special modifications or purity requirements.
Fields marked with an asterisk (*) are mandatory. It is helpful to include the peptide sequences in a standardized format (e.g., single-letter amino acid codes) and specify whether you need N-terminal acetylation, C-terminal amidation, or other modifications.
Choosing the Right Format for Your Research
Selecting between small-spot and large-spot arrays depends on your assay type and throughput needs. If you are screening a large library for binding partners or substrate preferences, the small-spot format with up to 1,400 peptides per membrane is likely the best choice.
For solution-based functional assays where you need more material per peptide, the large-spot format with about 110 peptides per membrane provides higher yield per spot. Consider your detection method, required sensitivity, and downstream applications when making this decision.
For more guidance on peptide handling and experimental design, explore our Peptide Glossary or use our Reconstitution Calculator to plan your experiments. If you are working with specific peptide sequences, our Interaction Checker can help identify potential cross-reactivities.
Conclusion
Peptide arrays offer a flexible platform for high-throughput screening, with formats ranging from high-density small-spot arrays (up to 1,400 peptides) to large-spot arrays (about 110 peptides) for solution assays. The maximum membrane size is 8 cm x 13 cm. By understanding these specifications, you can request a custom quote that matches your experimental requirements and accelerates your research.
