Key Takeaways
- •ABI Prism 7500 & FAST 7500
- •ABI Prism 7900 & FAST 7900
- •ABI Step One & Plus
- •CFX96 & CFX384 & CFX Duet (all versions)
- •DNA Engine Opticon 1 & 2
Guide to Free qPCR and RT-qPCR Master Mix Samples
Free qPCR samples contain enough reagents for up to 200 rxns (20 µL). These samples suit routine testing and research with options for probe or SYBR Green assays. Selection depends on assay type, starting material, and thermocycler.
Steps to Select Your Sample
First, pick the right sample based on your needs. Then, choose the sample size on the product page. Finally, add it to your cart.
Consider assay type such as probes or SYBR Green. Factor in starting material like DNA or RNA. Match your qPCR thermocycler for best results.
Probe Assay Options
Probe assays provide highly specific detection and allow multiple targets in co-detection. They adapt well to routine testing with versatile methods.
SYBR Green assays offer a cost-efficient choice for single target detection. They use simple assay design and rely on melt curve for specificity. These suit research purposes.
Supported Thermocyclers
Match your thermocycler to its signal normalization: ROX, low ROX, or no ROX. Use the list below if unsure. Contact support if your model lacks.
- ABI Prism 7500 & FAST 7500
- ABI Prism 7900 & FAST 7900
- ABI Step One & Plus
- CFX96 & CFX384 & CFX Duet (all versions)
- DNA Engine Opticon 1 & 2
- EONIS™ Q96/Q384
- Exicycler™ 96
- FastGene® qFYR
- iCycler iQ
- LightCycler® 96 & LightCycler® 480 (I & II)
- LightCycler Nano System
- LightCycler® PRO
- LineGene (all versions)
- MiniOpticon
- Mx3000P / Mx3005P/ Mx4000 with ROX normalization
- Mx3000P / Mx3005P/ Mx4000 without ROX normalization
- MyGo (all versions)
- QIAquant 96 & 384
- qTOWER & qTOWER³ & qTOWERiris
- QuantGene 9600
- QuantStudio (all versions)
- Rotor-Gene 2000/3000/6000/Q
- SmartCycler 1 & 2
- Swift Spectrum 96 & ProGene
For planning qPCR in broader research, check Volta's free peptide tools, including calculators for experiment setup.
ROX Options and Features
No ROX Probe Samples
- No ROX DNA Probe 5X MMx dTTP Sample: Carry-over prevention not included, kit converter available, inhibitor tolerance, bacterial target, multiplex ready, one-step conversion.
- No ROX Probe 2X MMx dTTP blue sample: Carry-over prevention not included, kit converter available.
- No ROX Probe 2X MMx dTTP sample: Carry-over prevention not included, kit converter available.
- No ROX Probe MMx UNG sample: Not compatible with carryover prevention.
- Ultra Probe 4X qPCR MasterMix sample: Carry-over prevention not included, one-step Takyon Ultra.
- Etheris Probe 2X qPCR Master Mix sample: Carry-over prevention not included.
Blue additive available separately. dUTP/UNG blend sold separately to enable carryover prevention by removing dUTP-containing DNA contamination from previous reactions.
ROX Probe Samples
Similar options exist:
- ROX Probe 5X MMx dTTP Sample: Carry-over prevention not included, kit converter.
- ROX Probe MMx dTTP blue sample: Carry-over prevention not included, kit converter.
- ROX Probe MMx dTTP sample: Carry-over prevention not included, kit converter.
- ROX Probe MMx UNG sample: Not compatible.
- Ultra Probe 4X qPCR MasterMix sample: Carry-over prevention not included, separate tube included, one-step Takyon Ultra.
- Etheris Probe 2X qPCR Master Mix sample: Carry-over prevention not included, separate tube included.
Notes match no ROX section.
Low ROX Probe Samples
- Low ROX Probe 5X MMx dTTP Sample: Carry-over prevention not included, kit converter.
- Low ROX Probe 2X MMx dTTP blue sample: Carry-over prevention not included, kit converter.
- Low ROX Probe 2X MMx dTTP sample: Carry-over prevention not included, kit converter.
- Low ROX Probe 2X MMx UNG sample: Not compatible.
- Ultra Probe 4X qPCR MasterMix sample: Carry-over prevention not included, separate tube included, one-step Takyon Ultra.
- Etheris Probe 2X qPCR Master Mix sample: Carry-over prevention not included, separate tube included.
SYBR Green Assay Options
SYBR options follow similar patterns for ROX, low ROX, no ROX.
ROX DNA SYBR
- ROX SYBR 2X MasterMix dTTP blue sample: Carry-over prevention not included, kit converter.
- Etheris SYBR 2X qPCR Master Mix blue sample: Not compatible, separate tube included.
Low ROX DNA SYBR
- Low ROX SYBR 2X MasterMix dTTP blue sample: Carry-over prevention not included, kit converter.
- Etheris SYBR 2X qPCR Master Mix blue sample: Not compatible, separate tube included.
No ROX DNA SYBR
- No ROX SYBR 2X MasterMix dTTP blue sample: Carry-over prevention not included, kit converter.
- Etheris SYBR 2X qPCR Master Mix blue sample: Not compatible.
One-Step Conversion and Notes
Convert any dTTP master mix for DNA detection to a one-step kit for RNA detection. Carryover prevention converter kit not compatible with 1-step RT-qPCR workflows. For 2-step, use first strand cDNA synthesis core kit.
Consult specialists for kit advice. See Peptide Glossary for related molecular biology terms in research.
Sample Conditions
Offer valid only for new qPCR customers. Valid once per customer. For research planning, visit latest peptide news.
Free samples support precise qPCR and RT-qPCR experiments with matched features.
