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Calculate step-by-step serial dilution series for laboratory and research use. Generates transfer volumes, diluent volumes, and resulting concentrations for each step.
At each step, a fixed transfer volume is taken from the previous tube and added to fresh diluent in the next tube. The concentration decreases by the dilution factor at each step.
| Step | Transfer Volume | Diluent Volume | Final Concentration | Total Volume |
|---|---|---|---|---|
| Stock | — | — | 100.000 mg/mL | — |
| 1 | 0.100000 mL | 0.900000 mL | 10.000 mg/mL | 1.000 mL |
| 2 | 0.100000 mL | 0.900000 mL | 1.000 mg/mL | 1.000 mL |
| 3 | 0.100000 mL | 0.900000 mL | 0.100000 mg/mL | 1.000 mL |
| 4 | 0.100000 mL | 0.900000 mL | 0.010000 mg/mL | 1.000 mL |
| 5 | 0.100000 mL | 0.900000 mL | 0.001000 mg/mL | 1.000 mL |
Educational purposes only
This calculator is provided for educational and informational purposes only. It does not constitute medical advice. Always consult a qualified healthcare professional and verify all calculations before any use.