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Calculate net charge at any pH and visualize the full titration curve with isoelectric point.
15 residues
-2.00
Net Charge at pH 7.4
3.55
Isoelectric Point (pI)
6
Ionizable Groups
| Group | pKa | Type | Charge at pH 7.4 |
|---|---|---|---|
| N-terminus | 9.69 | positive | +0.995 |
| C-terminus | 2.34 | negative | -1.000 |
| E (pKa 4.25) | 4.25 | negative | -0.999 |
| K (pKa 10.53) | 10.53 | positive | +0.999 |
| D (pKa 3.65) | 3.65 | negative | -1.000 |
| D (pKa 3.65) | 3.65 | negative | -1.000 |
Uses Henderson-Hasselbalch equation with standard pKa values. Actual charge may vary due to local electrostatic effects and post-translational modifications. For educational purposes.