Reconstitution
Definition
The process of adding a solvent (usually bacteriostatic water) to a lyophilized peptide to create a liquid solution for use.
What this means for research sourcing
Getting this right matters when preparing lyophilized research peptides for in vitro work. Volta ships compounds as lyophilized powder for reconstitution.
Related Terms in Preparation
Bacteriostatic Water
Sterile water containing 0.9% benzyl alcohol as a preservative. Used to reconstitute lyophilized peptides. Unlike sterile water, it inhibits bacterial growth and allows multi-dose use.
Sterile Water
Water that has been sterilized and contains no preservatives. Unlike bacteriostatic water, it does not inhibit bacterial growth and should be used for single-dose preparations only.
Lyophilization
The freeze-drying process used to convert peptide solutions into stable powders for long-term storage. Removes water under vacuum at low temperature, preserving the peptide's molecular structure. Lyophilized peptides can remain stable for years at -20°C.
Lyophilized
Freeze-dried. Peptides are commonly sold in lyophilized (powdered) form to preserve stability during storage. Must be reconstituted before use.
Vial
A small glass or plastic container used to store lyophilized or reconstituted peptides. Typically sealed with a rubber stopper and aluminum crimp cap.
Aliquot
A measured portion of a solution divided from a larger volume. Peptides may be aliquoted into single-use portions to avoid repeated freeze-thaw cycles.
Diluent
A liquid used to dilute or reconstitute a substance. For peptides, common diluents include bacteriostatic water and sterile saline.
Excipient
An inactive substance that serves as a carrier or stabilizer in a formulation. Mannitol is a common excipient in lyophilized peptide products.