target-validation

Peptide Pull-Down Assays for Antibody Target Validation

Peptide pull-down assays confirm antibody specificity by immobilizing bait peptides to capture target proteins from crude extracts. These methods use biotinylated peptides bound to streptavidin-coupled magnetic beads, allowing rigorous washing to ensure specific binding. Early validation saves time and resources in drug discovery by eliminating nonspecific binders.