B12 10mg
For in-vitro laboratory research only. Not for human or animal administration.
Batch #: VPB110100
Get Notified When Available
This product is currently out of stock. Enter your email and we'll notify you the moment it's back.
Research Use Only
For in-vitro laboratory research by qualified professionals only. Not for human or animal administration. Not a drug, food, cosmetic or dietary supplement. Not intended to diagnose, treat, cure, mitigate or prevent any disease. Batch-specific Certificates of Analysis available for all products.
B12 (Methylcobalamin) 10mg: overview
What the vial contains and what the material is, stated as specifications rather than as outcomes.
B12 (Methylcobalamin) supplied as a lyophilized powder in a sealed single-use vial containing 10 mg of material. B12 (Methylcobalamin): molecular formula C₆₃H₉₁CoN₁₃O₁₄P, molecular weight 1,344.4 g/mol, CAS 13422-55-4. Released to a specification of >99% purity by HPLC. Soluble in bacteriostatic water. Supplied for in-vitro laboratory research only. Not a drug, food or supplement. Not for human or veterinary use.
Volta does not provide dosing, administration or protocol guidance for any material listed.
B12 (Methylcobalamin) 10mg specifications
Every field the product record holds. A field with no value is omitted rather than printed as a dash.
- Fill
- 10mg
- Form
- Lyophilized powder
- CAS number
- 13422-55-4
- Molecular formula
- C₆₃H₉₁CoN₁₃O₁₄P
- Molecular weight
- 1,344.4 g/mol
- Solubility
- Soluble in bacteriostatic water
- Shelf life
- 24 months from date of manufacture
B12 analytical verification and batch documentation
What the purity figure on this page is, who measured what, and which of the two a reader is looking at.
Specification. Every batch is released to >99% purity by HPLC. That is a threshold Volta sets, and it is a promise rather than a measurement.
Measurement. No certificate for this compound is published on the site yet. A batch-specific Certificate of Analysis is available on request, and the batch history lists the ones already published. Until one is published for this material, the figure above is the release specification and nothing on this page is a laboratory result.
Checking a certificate. The batch number printed beside the price is derived from the compound code and the vial strength; the lot number on a certificate is transcribed from the document. They are produced independently, so comparing them is a real check. How to read one is set out in the quality and testing methodology page.
For in-vitro laboratory research by qualified professionals only. Not for human or animal administration. Not a drug, food, cosmetic or dietary supplement. Not intended to diagnose, treat, cure, mitigate or prevent any disease.
Vitamin B12 exists in several forms, and methylcobalamin is the one that functions directly as a cofactor without requiring intracellular conversion, which distinguishes it from cyanocobalamin in experimental work. It supports methionine synthase in the remethylation of homocysteine to methionine, the reaction that regenerates S-adenosylmethionine and links B12 status to methylation capacity generally. The cobalt-corrin structure makes it light-sensitive, so the amber vial and dark storage are functional requirements rather than convention.
- Released to a >99% purity specification by HPLC
- Lyophilized powder, 10mg per vial
- Soluble in bacteriostatic water
- For laboratory research use only
Frequently Bought Together
B12 (Methylcobalamin) 10mg
10mg
$21 USD
Bundle & Save
$105 USD
Save $5.25 USD
$144 USD
Save $14.40 USD
$188 USD
Save $28.20 USD
$262 USD
Save $52.40 USD
Click any product above to toggle selection
Related research materials
B12 (Methylcobalamin) 10mg: what is in the vial
The arithmetic specific to this 10mg vial, and what a milligram of B12 (Methylcobalamin) costs in each strength the catalogue carries. Concentrations are stated, not recommended.
Vial contents
10 mg
Lyophilised powder, reconstituted by the buyer
Cost of material
$2.10 / mg USD
CA$3 / mg in Canadian dollars
Concentration at each diluent volume
10 mg of dry material reaches these concentrations in the volumes below. A U-100 syringe marking is 0.01 ml by definition, so the last column is a unit conversion at each concentration rather than a quantity to use.
| Diluent added | Concentration | In 0.1 ml | Per U-100 unit |
|---|---|---|---|
| 1 ml | 10 mg/ml | 1 mg | 100 mcg |
| 2 ml | 5 mg/ml | 500 mcg | 50 mcg |
| 3 ml | 3.33 mg/ml | 333.3 mcg | 33.3 mcg |
| 5 ml | 2 mg/ml | 200 mcg | 20 mcg |
For a volume this table does not list, the reconstitution calculator takes any vial size and diluent volume.
B12 (Methylcobalamin) purity and identity: how the figure is measured
What >99% (HPLC) means, the masses an identity check has to land on, and the entries that make a certificate of analysis checkable rather than decorative.
Stated purity
>99% (HPLC)
Area percent of the main peak by reversed-phase HPLC
Average mass
1,344.4 g/mol
The figure an identity check has to land on
Identity by mass: the ions to expect
An electrospray source protonates the molecule rather than weighing it neutral, so a spectrum shows a series of charge states rather than the molecular weight itself. These are the m/z values 1,344.4 g/mol produces, and they are what a mass spectrum on a certificate for B12 (Methylcobalamin) has to match.
| Ion | Charge | Expected m/z |
|---|---|---|
| [M+H]+ | 1+ | 1,345.41 |
What a certificate for B12 (Methylcobalamin) should carry
A purity percentage on its own is not checkable. These are the entries that make one verifiable, and their absence is the most common weakness in a research-peptide certificate.
The chromatogram, not only the number
A stated area percent with no trace behind it cannot be read for the shape of the main peak or for what eluted beside it. The HPLC interpreter walks through what a trace shows.
Net peptide content, separately from gross mass
A lyophilised peptide is a salt, usually of trifluoroacetic or acetic acid, plus residual water. The vial's stated milligrams are gross; net peptide content is the fraction of that mass which is the molecule. The two differ by ten to twenty percent routinely, and only one of them is what the price is per milligram of. The net peptide content calculator converts between them.
The counterion, named
Which salt form the powder is in changes the net content and the pH the powder dissolves at. A certificate that never names it leaves both unknowable.
Water content, by a stated method
Loss on drying and Karl Fischer titration give different numbers, and a water figure with no method attached cannot be compared with anyone else's.
A laboratory and a report identifier
Without both, nothing on the document can be traced back to the laboratory that issued it. The red flag checker lists the rest.
Batch certificates are published as page images in the certificate library. The source PDFs are never served: a certificate is the most forgeable document a supplier publishes, and an editable copy carrying an accredited laboratory's letterhead is worth more to a counterfeiter than to a customer.
B12 (Methylcobalamin) storage and stability
Handling as the product record states it, followed by the degradation chemistry this particular sequence is and is not exposed to.
Handling
Store lyophilized peptide at -20°C in a dry, dark environment. Reconstitute in bacteriostatic water. Once reconstituted, store at 2-8°C and use within 30 days. Avoid repeated freeze-thaw cycles. Lyophilized powder is stable at room temperature for shipping and short-term storage.
A residue-level stability profile needs a primary sequence of standard amino acids. This compound's sequence carries modified or non-standard residues, so no finding is derived for it rather than one being estimated from a partial reading. The storage guide covers the general case.
B12 (Methylcobalamin) compared with DSIP and Epithalon
Pharmacological class, half-life, evidence grade, competition status and cost per milligram, side by side.
| Compound | Class | Half-life | Evidence | WADA | Cheapest per mg |
|---|---|---|---|---|---|
| DSIP | Sleep / Neuropeptide | ~7–8 minutes IV; longer SC | DPreclinical | Not listed | $4.6710mg vial |
| Epithalon | Anti-Aging / Telomere | Several hours | DPreclinical | Not listed | $3.4010mg vial |
| HCG | Hormonal / Reproductive | ~24-36 hours | AFDA Approved | Not listed | — |
| KPV | Anti-Inflammatory / Immune | ~2 hours (SC); shorter oral due to GI degradation | DPreclinical | Not listed | $3.9010mg vial |
| MOTS-c | Metabolic / Mitochondrial | Several hours; tissue effects may persist longer | DPreclinical | Not listed | $3.2010mg vial |
Evidence grades and half-lives are as recorded in the compound database, which cites its own sources on each compound page. Per-milligram prices are the cheapest strength each compound is currently listed at, in US dollars, and an out-of-stock note means that figure is not purchasable today. Cross-trial comparisons of efficacy are not comparisons: no head-to-head trial exists for most of these pairs.
B12 (Methylcobalamin) in Canada
Price in Canadian dollars, where the parcel ships from, and how long it takes.
Price in CAD
CA$30
The figure charged, not a converted estimate
Ships from
British Columbia
A domestic parcel, so no import clearance step
Transit
2 to 5 business days
After 1 to 2 business days of handling
Free standard shipping
Over CA$250
A bar set for this market, not converted from the US one
B12 (Methylcobalamin) 10mg ships from British Columbia to Canadian addresses, so the parcel never crosses a border. That removes the failure a Canadian buyer of research peptides is usually weighing: an inbound international shipment can be held for import clearance or seized, and a domestic one has no clearance step to be held at.
Shipping is quoted live against the delivery address at checkout rather than estimated here, and both the standard and express tiers show their price and transit window before a payment method is chosen. The figure the page shows is the figure the rail charges: all three settlement rails price shipping through the same functions the quote does.
The Canadian figure above is not a loose conversion. Each product's US dollar base is chosen so that the live conversion lands on the Canadian shelf price set for this market, and the result is pushed up to a whole dollar rather than left carrying cents, so one figure serves the page, the feed and every payment rail. See the shipping policy for carriers and cut-off times, and the legal position on research peptides in Canada for the regulatory picture.
Vitamin B12 Mechanism of Action
Vitamin B12 is not a peptide. It is cobalamin, a cobalt-containing corrinoid and the largest and most structurally complex of the vitamins, and it is the only one containing a metal ion at its centre. It sits in this catalogue for commercial reasons rather than chemical ones, and almost everything said elsewhere here about peptide handling and certificate interpretation does not apply to it.
In human metabolism it is a cofactor for exactly two enzymes, and that narrowness is what makes its biochemistry unusually tractable. Methylcobalamin is the cofactor for methionine synthase, which converts homocysteine to methionine and in doing so regenerates tetrahydrofolate; a deficiency therefore raises homocysteine and traps folate in an unusable form. Adenosylcobalamin is the cofactor for methylmalonyl-CoA mutase, which handles propionyl-CoA from odd-chain fatty acid and certain amino acid catabolism; a deficiency raises methylmalonic acid.
Those two consequences are why methylmalonic acid and homocysteine are the functional markers of B12 status and are more informative than serum B12 itself. Serum concentration measures what is circulating, including inactive analogues bound to haptocorrin; the metabolites measure whether the two enzymes are actually working. That distinction is the single most practically useful thing about B12 biochemistry.
Absorption via intrinsic factor
Dietary B12 binds intrinsic factor secreted by gastric parietal cells and is absorbed in the terminal ileum through the cubam receptor, a route that fails in pernicious anaemia and after certain gastric or ileal surgery.
Conversion to active forms
Cobalamin is processed intracellularly, with MMACHC among the proteins handling this step, into the two coenzyme forms the body actually uses.
Methylcobalamin and methionine synthase
Cofactor for the conversion of homocysteine to methionine, which also regenerates tetrahydrofolate. Deficiency raises homocysteine and traps folate.
Adenosylcobalamin and methylmalonyl-CoA mutase
Cofactor for handling propionyl-CoA from odd-chain fatty acids and certain amino acids. Deficiency raises methylmalonic acid.
Functional markers
Because those are the only two reactions, methylmalonic acid and homocysteine report on enzyme function directly, where serum B12 reports only on what is circulating.
Vitamin B12 Research Findings
B12 has a genuine clinical evidence base, which distinguishes it from most compounds in this catalogue. Each entry names the setting, and the distinction between correcting deficiency and supplementing sufficiency is maintained throughout.
Oral dose comparison for neuropathic outcomes in deficiency
A trial compared oral vitamin B12 at two dose levels on neuropathic outcomes, addressing the practical question of how much is needed rather than whether repletion works at all. Correcting a deficiency is the setting where the evidence is strongest.
Phase 3 trialMaternal supplementation improved outcomes in a vegetarian population
Supplementation of predominantly vegetarian pregnant women improved measured outcomes, in a population where dietary intake is genuinely limited because B12 occurs in animal-derived foods and in fortified products.
Phase 3 trialComparative review of natural and synthetic forms
A comprehensive review compared the forms of B12 used in supplementation, which is the question most often asked about this compound and most often answered with marketing rather than evidence.
ObservationalRelationship between B12, folate, homocysteine and haemoglobin
Population work examined methylcobalamin, folate, homocysteine and haemoglobin together, which is the correct way to read B12 status because the folate and homocysteine relationships are part of the same biochemistry.
ObservationalStructural basis of intracellular cobalamin processing
Biophysical work on how cobalamin variants modulate the conformation and oligomerisation of MMACHC addressed the protein that processes cobalamin inside the cell, which is where inherited disorders of B12 handling arise.
In vitroImmunomodulatory effects of B12 derivatives on macrophages
Work reported immunostimulatory and immunomodulatory effects of vitamin B12 derivatives on macrophages, extending interest beyond the two classical cofactor roles into immune signalling.
In vitroNeurological presentation of deficiency documented
A case report described sensory ataxia associated with B12 deficiency, illustrating that the neurological consequences of deficiency can present without the anaemia usually associated with it.
ObservationalVitamin B12 Molecular Information
| Class | Cobalamin, a cobalt-containing corrinoid. Not a peptide |
| Molecular Weight | 1355.4 g/mol for cyanocobalamin |
| Molecular Formula | C63H88CoN14O14P for cyanocobalamin |
| CAS Number | 68-19-9 for cyanocobalamin |
| Central Atom | Cobalt, coordinated within a corrin ring |
| Common Forms | Cyanocobalamin, methylcobalamin, hydroxocobalamin, adenosylcobalamin |
| Active Coenzyme Forms | Methylcobalamin and adenosylcobalamin only |
| Enzyme Cofactor For | Methionine synthase and methylmalonyl-CoA mutase |
| Functional Status Markers | Methylmalonic acid and homocysteine |
| Appearance | Dark red crystalline powder, the colour coming from the cobalt corrin complex |
The Form Question, Answered Carefully
Methylcobalamin is widely marketed as superior to cyanocobalamin on the reasoning that it is one of the two forms the body actually uses, so no conversion is required. The reasoning is real, and the conclusion is weaker than it sounds.
The body interconverts these forms. Cyanocobalamin is processed intracellularly to both active coenzymes, and it is the form used in most of the clinical trial literature, including the studies that established B12 repletion works. Hydroxocobalamin has a longer retention time than either and is preferred in some clinical settings for that reason rather than for its being closer to an active form.
The honest summary is that all forms correct deficiency, that cyanocobalamin has the largest evidence base by a wide margin, and that the case for methylcobalamin rests more on mechanistic reasoning than on comparative trials. Anyone claiming a large clinical difference is going beyond what the comparative literature supports.
Deficiency and Supplementation Are Different Questions
The evidence that correcting B12 deficiency helps is strong, unambiguous and decades old. Deficiency causes megaloblastic anaemia and a neurological syndrome including peripheral neuropathy and subacute combined degeneration of the cord, and repletion addresses both, with the neurological recovery being incomplete if treatment is delayed.
The evidence that supplementing people who are not deficient produces benefit is quite different and much weaker. These are separate questions and they are constantly merged in marketing for injectable B12, which borrows the strength of the deficiency literature to support a use that literature never examined.
Populations where deficiency is genuinely common are well defined: strict vegetarian and vegan diets, pernicious anaemia, atrophic gastritis, metformin use, and post-surgical loss of the ileal absorption site. Outside those, sufficiency is the usual finding.
Why Serum B12 Is a Poor Test
Serum B12 measures total circulating cobalamin, and a substantial fraction of that is bound to haptocorrin and unavailable to tissues. It also includes inactive corrinoid analogues that the assay does not distinguish from the real thing. The result is a test with wide indeterminate ranges and a known false-negative problem.
Methylmalonic acid and homocysteine are functional markers: they rise when the two B12-dependent enzymes are not working, which is the thing actually of interest. Methylmalonic acid is the more specific of the two, since homocysteine also rises in folate and B6 deficiency and in renal impairment.
This matters practically because it explains why someone can have a normal serum B12 and a genuine functional deficiency, which is one of the more common sources of confusion around this vitamin.
Handling and Analytical Considerations
B12 is light-sensitive, and that is a chemical property of the corrin system rather than general caution: photolysis of the cobalt-carbon bond degrades methylcobalamin and adenosylcobalamin particularly readily. Amber containers and dark storage are functional requirements for this compound rather than conventions carried over from peptide handling.
As a small molecule, its certificate should carry small molecule specifications: identity by spectroscopic method, assay by HPLC against a reference standard, and residual solvent analysis. The characteristic red colour and its ultraviolet-visible absorption spectrum are also a useful identity check available with basic equipment.
Vitamin B12 Research FAQ
Vitamin B12 in Summary
B12 is cobalamin, a cobalt corrinoid rather than a peptide, and a cofactor for exactly two human enzymes: methionine synthase and methylmalonyl-CoA mutase. Those two roles explain why methylmalonic acid and homocysteine report status better than serum B12 does.
The evidence for correcting deficiency is strong and long established; the evidence for supplementing sufficiency is not, and the two are routinely merged. All common forms correct deficiency, with cyanocobalamin carrying by far the largest trial evidence base.
Scientific References
Primary literature and public trial registries only. No supplier or retailer pages are cited.
- 1Vitamin B12: A Comprehensive Review of Natural vs Synthetic Forms of Consumption and SupplementationBehringer CR, Kulkarni A, et al. · Cureus · 2025
- 2Efficacy of Oral Vitamin B-12 at 1000 micrograms Compared with 2000 micrograms on Neuropathic OutcomesMansour A, Amrollahi Bioky A, et al. · The Journal of Nutrition · 2026
- 3Maternal supplementation of vitamin B12 in predominantly vegetarian pregnant women improves outcomesNagpal J, Mathur M, et al. · BMJ Paediatrics Open · 2026
- 4Gender differences in methylcobalamin, folate, homocysteine, and hemoglobin levelsKuchipudi JD, Menon J, et al. · Journal of Alzheimer's Disease · 2026
- 5Modulation of conformational features and oligomerization of MMACHC by cobalamin variantsLongo L, Costa MA, et al. · European Biophysics Journal · 2026
- 6Immunostimulatory and Immunomodulatory Effects of Vitamin B12 Derivatives on MacrophagesBaşkan ÖM, Ercan S, et al. · Cell Biochemistry and Biophysics · 2026
- 7Sensory ataxia associated with vitamin B12 deficiency and hypocalcaemiaHashimoto T, Kubota M, et al. · BMJ Case Reports · 2026
Disclaimer
All articles and product information provided on this website are for informational and educational purposes only. The products offered on this website are furnished for in-vitro studies only. These products are not medicines or drugs and have not been approved by the FDA to prevent, treat or cure any medical condition, ailment or disease.
B12 (Methylcobalamin) 10mg: frequently asked questions
Answered from the product record and the certificate file. Volta does not answer questions about administration, dosing or protocols.
What is supplied in a 10 mg vial of B12?
A sealed single-use vial containing 10 mg of B12 (Methylcobalamin) as a lyophilized powder. Soluble in bacteriostatic water. No diluent, syringe or other supply is included.
Is B12 supplied for human use?
No. For in-vitro laboratory research by qualified professionals only. Not for human or animal administration. Not a drug, food, cosmetic or dietary supplement. Not intended to diagnose, treat, cure, mitigate or prevent any disease. Volta does not provide dosing, administration or protocol guidance for any material listed.
What purity is this B12 released to?
>99% by HPLC. That figure is a release specification, a threshold Volta sets for every batch, and it is not the same kind of statement as a purity measured by a named laboratory for a named lot.
Is there a certificate of analysis for this B12 vial?
A batch-specific Certificate of Analysis is available for this product on request. It is not published on the site yet: the batch history on the quality page lists the certificates already published, and this vial is covered by the release specification until its own is added there.
How is B12 identified?
CAS 13422-55-4, molecular formula C₆₃H₉₁CoN₁₃O₁₄P, molecular weight 1,344.4 g/mol. Those identifiers are what an incoming-goods check compares a certificate against, and they are stated here so the comparison can be made before ordering.
How should B12 be stored before reconstitution?
Store lyophilized peptide at -20°C in a dry, dark environment. Reconstitute in bacteriostatic water. Once reconstituted, store at 2-8°C and use within 30 days. Avoid repeated freeze-thaw cycles. Lyophilized powder is stable at room temperature for shipping and short-term storage.
Where does this ship from?
British Columbia, Canada. Canadian orders are domestic, so they clear no customs and pay no import duty. International orders ship from the same facility.
Related Research News
Best Peptide Affiliate Program in 2026: 8 Programs Compared by What They Actually Pay
10% flat is the going rate in research peptides, and almost every page quoting it calls it competitive. Eight programs compared on the only four things that decide what you are paid: the rate, the base it is applied to, how long attribution lasts, and when the money actually arrives.
Tesamorelin vs Ipamorelin: Human Evidence, Doses, Safety
Tesamorelin holds FDA approval for HIV-associated lipodystrophy; ipamorelin has none. This page maps the published human dosing data for each and marks the gaps.
Semaglutide News: New Meta-Analysis Highlights Weight and Inflammatory Benefits
A recent systematic review and meta-analysis examines the effects of subcutaneous semaglutide on weight loss and inflammatory markers in adults with overweight or obesity without diabetes. Early reports suggest meaningful reductions in body weight alongside improvements in inflammatory biomarkers, offering new context for peptide researchers and industry professionals.
Explore Research
Peptide Tools
More from the research catalogue
Every compound below has its own specification, batch number and certificate page, whether or not a vial is in stock today. Supplied for laboratory research use only.
Related research compounds
- Cerebrolysin 60mgrestocking
- Dihexa 10mgrestocking
- DSIP 10mg
- Epithalon 10mg
- HGH Fragment 176-191 5mgrestocking