mass spectrometry
5 articles tagged with ‘mass spectrometry’
Peptide Testing in Canada: HPLC, Mass Spec and Reading a COA
Every vendor says its material is pure, and almost nobody who reads the claim can evaluate it. What reversed-phase HPLC and mass spectrometry each actually measure, the seven fields a Certificate of Analysis has to carry, the four things it cannot establish, and the one verification step that does not depend on trusting the seller.
Identity Is the Test Almost Nobody Runs
Rearrange TB-500's seven residues and there are 1,260 arrangements that all weigh exactly the same. Purity gets reported constantly and identity almost never, so here is what each method can and cannot establish about whether a vial holds the sequence on the label.
BPC-157 10mg: Sequence, Analytical Identity and the Shape of the Literature
BPC-157 is a 15-residue synthetic peptide with no cysteine, methionine or tryptophan. Its sequence and registry data, what HPLC and mass spectrometry confirm on a lot, and why its published literature has to be read with its study design in view.
TB-500 and Thymosin Beta-4: Identity, Actin Binding and the State of the Literature
TB-500 is the seven-residue actin-binding fragment of thymosin beta-4, and the name is used elsewhere for three other molecules. How the certificate identifies the one in the vial, what G-actin sequestration means at the molecular level, and which parts of the literature carry retractions.
How to Read a Certificate of Analysis (COA): The Complete Guide for Peptide Researchers
A working guide to interpreting every section of a peptide Certificate of Analysis, from HPLC chromatograms and mass spec data to spotting the red flags that separate credible documentation from recycled paperwork.
